/* Desens.hoc
This file is used to demonstrate activation and desensitization of the dAMPA
receptor as well as recovery from desensitization.
Activation and desensitization are demonstrated with a single pulse of glutamate
ranging from 0.25 to 10ms in duration.
Recovery from desensitization is demonstrated with two 1 ms. pulses of glutamate
with an isi ranging from 25 to 400 ms.
*/

create soma
soma {
nseg = 1
L = 25
diam = 25
}

load_proc ("nrnmainmenu")

forall insert pas
forall g_pas=1/70000
forall Ra=110
global_ra=110
forall e_pas=-65


access soma
objectvar stim1
stim1 = new glutamate(.50)
stim1.DC_Level = 1
stim1.DC_Delay = 10
stim1.DC_Off = 500

objectvar chanN1
soma chanN1 = new dAMPA_R(.50)
// Manni's kinetics
chanN1.gmax = 0.0013
chanN1.RBd = 0.4
chanN1.RBDr = 0.03
chanN1.RBb = 0.004
chanN1.RB2d = 5e-05
chanN1.RB2Dr = 0.0002

objectvar clamp
soma clamp = new VClamp(0.5)
clamp.dur[0] = 1200
clamp.amp[0] = -65


tstop = 1200
dt = .025
steps_per_ms = 5

v_init = 65
xopen("AMPA_DESENS_NEW.ses")